● The analysis of large-scale gene expression correlated to the phase changes of the migratory locust. PNAS 101: 17611-17615 (2004)(PDF)

The basic description about 12161 Unigenes

Annotation results by a series of blast analysis against NCBI_NR, NCBI_NT and Swiss Prot database.

Gene Onotology (GO) annotation of 12161 unigenes

KEGG pathway analysis of 12161 unigenes

Classification of the unigenes into COG (Cluster of Orthologous Groups) functional categories.

Orthologous gene analysis against five species: Apis Mellifera; Anopheles Gambiae; Drosophila Melanogster; Bombyx Mori and Caenorhabditis Elegans

Codon Usage table created for locusta migratoria

LocustDB Introduction

The migratory locust (Locusta migratoria) is an orthopteran pest and a representative member of hemimetabolous insects. Its transcriptomic data provide invaluable information for molecular entomology study of the insect and pave a way for comparative studies of other medically, agronomically, and ecologically relevant insects. We have developed this first transcriptomic database of the locust (LocustDB), building necessary infrastructures to integrate, organize, and retrieve data that are either currently available or to be acquired in the future. It currently hosts 45,474 high quality EST sequences from the locust, which were assembled into 12,161 unigenes. It, through user-friendly web interfaces, allows investigators to freely access sequence data, including homologous/orthologous sequences, functional annotations, pathway analysis, and codon usage, based on conserved orthologous groups (COG), gene ontology (GO), protein domain (InterPro), and functional pathways (KEGG). LocustDB also provides information from comparative analysis based on data from the migratory locust and five other invertebrate species, such as the silkworm, the honeybee, the fruitfly, the mosquito and the nematode. LocustDB starts with the first transcriptome information for an orthopteran and hemimetabolous insect and will be extended to provide a framework for incorporation of in-coming genomic data of relevant insect groups and a workbench for cross-species comparative studies. (Figure 1.)

Unigene	No.
Total	12,161
Singlets	7,611
With 2 or more ESTs	4,550
With 5 or more ESTs	1,649
With 10 or more ESTs	797
With 20 or more ESTs	371
With 50 or more ESTs	109
With 100 or more ESTs	39

Data	Total	Gregarious				Solitarious
		Body	Head	Midgut	H-Leg	Head	Midgut	H-Leg
Acquired	76,012	18,480	10,996	10,291	8,981	10,675	9,628	6,961
Analyzed	45,449	12,385	5,724	6,831	4,620	5,361	6,062	4,466
Mit-elated	18,545	2,677	3,763	3,317	2,976	2,796	1,834	1,182
Others	12,018	3,418	1,509	143	1,385	2,796	1,732	1,313